Optimising rAAV Purification: Thermo Scientific POROS™ CaptureSelect™ AAVX Resin Delivers High Capacity and Efficiency for Gene Therapy Manufacturing
Recombinant adeno-associated virus (rAAV) is a key tool for gene therapy, with applications ranging from rare diseases to widespread conditions like hemophilia. Production methods have standardized, with HEK293 cell transfection yielding high titers necessary for large-scale treatments. Thermo Scientific POROS™ CaptureSelect™ AAVX resin has become a commonly used approach for rAAV purification.
This study evaluates dynamic binding capacity (DBC) across different AAV serotypes and process conditions to optimize productivity, balancing efficiency for both clinical and commercial manufacturing scales.
The portfolio of resins helps to:
- High binding capacity: AAVX resin achieves >1E15 capsids/mL at residence times ≥ 0.5 min for AAV2.
- Optimized productivity: Shorter load residence times (≤ 0.5 min) enhance performance.
- Clinical manufacturing: High DBC allows flexibility in process conditions with small column volumes.
- Large-scale production: For high titers and large bioreactors, columns of 20–30 cm diameter optimize processing and resin use.
